清华大学一篇论文，证明了动物细胞也具有全能性？

清华大学一篇论文，证明了动物细胞也具有全能性？

在自然杂志的网站上，找到了up提到的这篇论文，标题为：Induction of mouse totipotent stem cells by a defined chemical cocktail，粗译一下：通过确定的化学混合物诱导小鼠全能干细胞

来看一下这篇文章的Abstract：

In mice, only the zygotes and blastomeres from 2-cell embryos are authentic totipotent stem cells (TotiSCs), capable of producing all the differentiated cells in both embryonic and extraembryonic tissues and forming an entire organism.

However, it remains challenging whether and how TotiSCs, representing the very beginning of a life, can be established in vitro in the absence of germline cells. Here, we demonstrate induction and long-term maintenance of TotiSCs from mouse pluripotent stem cells (PSCs) by a combination of three small molecules, TTNPB, 1-Azakenpaullone, and WS6. These cells, which we designated as ciTotiSCs (chemically induced totipotent stem cells), resembled mouse totipotent 2C-embryo stage cells at transcriptome, epigenome and metabolome level.

In addition, ciTotiSCs exhibited bidirectional developmental potentials and were able to produce both embryonic and extraembryonic cells in vitro and in teratoma. Furthermore, following injection into 8-cell embryo, ciTotiSCs contributed to both embryonic and extraembryonic lineages with high efficiency.

Our chemical approach for TotiSCs induction and maintenance provides a defined in vitro system to manipulate and understand totipotent state towards creating life from non-germline.

简单机翻：

在小鼠中，只有来自 2 细胞胚胎的受精卵和卵裂球才是真正的全能干细胞 (TotiSCs)，它们能够在胚胎和胚胎外组织中产生所有分化的细胞并形成一个完整的有机体1。然而，在没有生殖细胞的情况下，是否以及如何在体外建立代表生命开始的 TotiSCs 仍然具有挑战性。

在这里，我们展示了通过三种小分子 TTNPB、1-Azakenpaulllone 和 WS6 的组合对小鼠多能干细胞 (PSCs) 的 TotiSCs 的诱导和长期维持。我们将这些细胞称为 ciTotiSCs（化学诱导的全能干细胞），在转录组、表观基因组和代谢组水平上类似于小鼠全能 2C 胚胎期细胞。此外，ciTotiSCs 表现出双向发育潜力，能够在体外和畸胎瘤中产生胚胎和胚胎外细胞。

此外，在注射到 8 细胞胚胎中后，ciTotiSCs 对胚胎和胚胎外谱系都有高效的贡献。我们用于 TotiSCs 诱导和维持的化学方法提供了一个明确的体外系统来操纵和理解全能状态，从而从非生殖细胞中创造生命。

原文在此：https://www.nature.com/articles/s41586-022-04967-9#article-comments